The first session will concentrate on the well-established methods of classical transgenesis and gene-targeting via embryonic stem cells, and will include examples of how to produce a range of mutation types; for example over-expression models, gene knock-outs and point mutations, both constitutive and conditional.
The second session will address more recent developments, such as the use of gene-editing nucleases, their advantages and limitations, and novel methods of gene transfer.
Presentation
Date: Thursday, 27th June 2024
Time: 9:30 – 13:00
Place: Fèlix Serratosa Auditory (Parc Científic de Barcelona)
Registration: Send an email before 21st June to phd@irbbarcelona.org with the subject "Registration Engineering genetic mutations in the mouse", indicating:
- Name and surname
- Research Group
- Job Position (PhD, Postdoc, BsC/MsC student, Lab Technician, etc)
A confirmation email will be sent to all participants.
The workshop is offered exclusively to members of IRB Barcelona and has limited places.
If you are registered to participate and finally cannot attend, please send an email to phd@irbbarcelona.org to give others the opportunity.
Programme
09:30 - 11:10 – 'Traditional' methods of transgenesis and gene targeting
11:10 – 11:30 – Break
11:30 – 13:00 – Gene editing using sequence specific nucleases. Current and future trend
Speakers
Mouse Mutant Core facility Manager