The course will cover a range of genomics and transcriptomics applications currently offered by the Functional Genomics Core Facility (FGCF). This year, we will pay special attention to the new field of spatial biology, as well as single cell transcriptomics and epigenomics.
Presentation
After last year’s focus on basic technological topics related to Next-Generation Sequencing technologies, our 2022 advanced course dives deeper into common and novel library structures, Illumina’s 2-channel sequencing by synthesis technology, sample preparation guidelines, and sample preparation troubleshooting. There will be plenty of time to discuss other technologies, address your questions, and discuss your research plans.
Whether genomics is a new topic for you, or you are already participating in ongoing projects, this course will bring you up to speed with the available tools and methodologies to move your research forward.
After the course, you should be able to:
Recognise genomics as a scientific topic and a global market.
Identify different Next-Generation Sequencing (NGS) technologies.
Understand that high quality genomics and transcriptomics results start with experimental design.
Understand sample quality metrics.
Understand the type of data obtained from an NGS experiment.
Identify advantages and disadvantages of currently available technologies.
Anticipate challenges and bottlenecks in your genomics/transcriptomics projects.
Apply sound practices when designing experiments, analysing data, or interpreting the results.
Employ next-generation multiplexed CRISPR screens in combination with single-cell transcriptomics as a means to elucidate gene function at scale.
Speaker: Freddy Monteiro
Functional Genomics Core Facility Manager
Audience: All IRB Barcelona members are welcome (previous registration requested).
Date and Time: Thursday 17 March, 09:30-17:15
Place: Fèlix Serratosa Room (Cluster I Building)
Level: Content is adapted to all audiences with a keen interest in genetics, genomics and biotech.
Sponsor:
Sponsored Seminar by Merck.
“CRISPR-based genome editing at single cell resolution”.
Registration: Fill in the registration form here before 13 March.
If you are registered to participate and finally cannot attend, please send an e-mail to phd@irbbarcelona.org in order to give others the opportunity.
Programme
Final Schedule
09:30-10:00 Course introduction
10:00-10:45 A brief history of high-throughput sequencing and its applications
11:00-11:30 Coffee break. Mix and Match of interests and expectations.
11:30-11:45 Library preparation and sequencing on Illumina platforms.
12:00-12:45 The IRB Barcelona Functional Genomics Core Facility: Expertise and workflows.
13:00-13:20 Keys for successful transcriptomics experiments.
13:30-14:30 Lunch break
14:30-15:15 TrascriptomicsToday. From whole tissue to single-cell and back to tissue at higher dimensions.
15:30-16:30 Merck Sponsored Seminar
CRISPR-based genome editing at single cell resolution
Speakers:
M. Zulfiquer Hossain, Ph.D.
Technical Application Scientist. MilliporeSigma. Merck KGaA
Stephen Hague, PhD.
Science & Technology Advisor.
10x Genomics
Join the seminar using this link
16:45-17:00 Course wrap up and feedback.